《植物生理学报》 2013, 49(12): 1442-1446

化学诱变剂和⁶⁰Co处理对大麦小孢子低氮胁迫培养的影响

陆瑞菊^1,2,*, 陈志伟^1,2,*, 何婷^1,2, 徐红卫^1,2, 杜志钊^1,2, 高润红^1,2, 王亦菲^1,2, 邹磊^1,2, 郭桂梅^1,2, 卜姝明³, 黄亦辰³, 刘成洪^1,2,**, 黄剑华^1,2,**

¹上海市农业科学院生物技术研究所, 上海201106; ²上海市农业遗传育种重点实验室, 上海201106; ³上海海洋大学水产与生命学院, 上海201306

通信作者：陆瑞菊；E-mail: sw1@saas.sh.cn, chliu001@163.com；Tel: 021-62201032

摘　要：

以大麦品种‘花30’为材料, 用不同剂量化学诱变剂(EMS和PYM)处理大麦游离小孢子, 不同剂量⁶⁰Co辐照处理大麦离体穗和干种子, 比较其对在低氮胁迫下小孢子培养诱导愈伤组织产量和绿苗分化数量的影响。结果表明, EMS处理离体小孢子和⁶⁰Co辐照干种子明显比PYM处理小孢子和⁶⁰Co辐照离体穗的培养效果好。

关键词：大麦; 小孢子培养; 诱变

收稿：2013-10-28   修定：2013-11-25

资助：大麦青稞产业技术体系(CARS-05)、上海市种业发展项目[沪农科种字(2012)第7号]、上海市科委基础重点项目(12JC1407800)

Effects of Chemical Mutagen and ⁶⁰Co Treatments on Microspore Culture of Barley under Low-Nitrogen Stress

LU Rui-Ju^1,2,*, CHEN Zhi-Wei^1,2,*, HE Ting^1,2, XU Hong-Wei^1,2, DU Zhi-Zhao^1,2, GAO Run-Hong^1,2, WANG Yi-Fei^1,2, ZOU Lei^1,2, GUO Gui-Mei^1,2, BU Shu-Ming³, HUANG Yi-Chen³, LIU Cheng-Hong^1,2,**, HUANG Jian-Hua^1,2,**

¹Biotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; ²Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai 201106, China; ³College of Fishery and Life Science, Shanghai Ocean University, Shanghai 201306, China

Corresponding author: LU Rui-Ju; E-mail: sw1@saas.sh.cn, chliu001@163.com; Tel: 021-62201032

Abstract:

To investigate the effects of chemical mutagens and ⁶⁰Co on callus yield and green plantlet production through microspore culture under low-nitrogen stress, we used the barley (Hordeum vulgare) variety ‘Hua 30’ as the tested material, used two chemical mutagens, ethylmethylsulfone (EMS) and pingyangmycin (PYM), with different concentrations to treat the microspores, and used ⁶⁰Co with different doses to treat young spikes and seeds respectively. The results showed that EMS treatment of microspores or ⁶⁰Co treatment of seeds were much better than PYM treatment of microspores or ⁶⁰Co treatment of young spikes for callus induction and green plantlet differentiation.

Key words: Hordeum vulgare; microspore culture; mutation